PRKCSH antibody

Catalog No. ABIN317664

Product Details anti-PRKCSH Antibody

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKCSH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of Glucosidase II beta protein.(region surrounding Lys117)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PRKCSH

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Alternative Name: Glucosidase 2 Subunit beta (PRKCSH Products)

Synonyms: AGE-R2 antibody, G19P1 antibody, PCLD antibody, PKCSH antibody, PLD1 antibody, 80K-H antibody, VASAP-60 antibody, zgc:55653 antibody, prkcsh antibody, MGC75615 antibody, MGC82696 antibody, PRKCSH antibody, protein kinase C substrate 80K-H antibody, protein kinase C substrate 80K-H L homeolog antibody, phospholipase D1 antibody, PRKCSH antibody, Prkcsh antibody, prkcsh antibody, prkcsh.L antibody, Bm1_42055 antibody, LOC100304710 antibody, LOC100526729 antibody, PLD1 antibody

Background: Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains alpha and beta subunits (Glucosidase IIalpha and Glucosidase IIbeta). The alpha and beta subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIalpha is the catalyitc core of the enzyme and can function independently of the beta subunit. The sequence of Glucosidase IIbeta encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIbeta is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIbeta was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIbeta retains the complete complex in the ER.Synonyms: 80K-H protein, G19P1, Glucosidase II beta, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain

Molecular Weight: approx. 59 kDa

Gene ID: 5589

NCBI Accession: NP_001001329

UniProt: P14314

Pathways: Cellular Glucan Metabolic Process, Methionine Biosynthetic Process