ID4 antibody

Catalog No. ABIN317715

The Rabbit Polyclonal anti-ID4 antibody (ABIN317715) specifically detects ID4 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ID4 antibody (ABIN317715)

Target: ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ID4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ID4 Antibody

Specificity: This antibody detects endogenous levels of ID4 / BHLHB27 protein (region surrounding Ser5).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-chromatography

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ID4

Target: ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))

Alternative Name: ID4 / BHLHB27

Background: Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate the expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins (encoded by Id-related genes) also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (referenced from entrez gene).Synonyms: Class B basic helix-loop-helix protein 27, DNA-binding protein inhibitor ID-4, Inhibitor of DNA binding 4

Molecular Weight: approx. 24 kDa

Gene ID: 3400

NCBI Accession: NP_001537

UniProt: P47928