NTSR1 antibody

Catalog No. ABIN317760

Product Details anti-NTSR1 Antibody

Target: NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NTSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: NTR1 antibody detects endogenous levels of NTR1 protein.(region surrounding His218)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NTSR1

Target: NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))

Alternative Name: Neurotensin Receptor 1 (NTSR1 Products)

Synonyms: NTSR1 antibody, Ntsr antibody, NT-1R antibody, NTR-1 antibody, NTR1 antibody, NTR antibody, neurotensin receptor 1 antibody, neurotensin receptor 1 (high affinity) antibody, NTSR1 antibody, Ntsr1 antibody

Background: Neurotensin (NT) initiates an intracellular response by interacting with the G protein-coupled receptors NTR1 (NTS1 receptor, high affinity NTR) and NTR2 (NTS2 receptor, levocabastine-sensitive neurotensin receptor), and the type I receptor NTR3 (NTS3 receptor, sortilin-1, Gp95). NT has a wide distribution in regions of the brain and in peripheral tissues where NT receptors can contribute to hypotension, hyperglycemia, hypothermia, antinociception and regulation of intestinal motility and secretion. HL-60 cells express NTR1, which can couple to Gq, Gi/o, or Gs. Alternative splicing of rat NTR2 can generate a 5-transmembrane domain variant isoform that is co-expressed with the full-length NTR2 throughout the brain and spinal cord. NTR3 activation in the murine microglial cell line N11 induces MIP-2, MCP-1, IL-1β and TNFα in an ERK1/2 and Akt kinase-dependent manner.Synonyms: High-affinity levocabastine-insensitive neurotensin, NT-R-1, NTR1, NTRR, NTSR1

Molecular Weight: approx. 36 kDa

Gene ID: 4923

NCBI Accession: NP_002522

UniProt: P30989