PAK4 antibody

Catalog No. ABIN317882

The Rabbit Polyclonal anti-PAK4 antibody is suitable to detect PAK4 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for PAK4 antibody (ABIN317882)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAK4 Antibody

Specificity: PAK4/5/6 antibody detects endogenous levels of PAK4/5/6 protein.(region surrounding Val469)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % Sodium Azide as preservative, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4

Background: Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.Synonyms: KIAA1142, KIAA1264, PAK 4, PAK-4, PAK-5, PAK-5, PAK-6, PAK-7, PAK5, Serine/threonine-protein kinase PAK 4, Serine/threonine-protein kinase PAK 6, Serine/threonine-protein kinase PAK 7, p21-activated kinase 4, p21-activated kinase 5, p21-activated kinase 6, p21-activated kinase 7

Molecular Weight: approx. 80 kDa

Gene ID: 10298

NCBI Accession: NP_001014831

UniProt: O96013

Pathways: RTK Signaling