PAK4 antibody (AA 23-72)

Catalog No. ABIN6746289

Product Details anti-PAK4 Antibody

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Binding Specificity: AA 23-72

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio), Bat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Rat PAK4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa23-72 of rat Pak4 (B5DF62, NP_001099708). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Galago, Xenopus (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PAK4 antibody (ABIN6746289)

Marte, Downward: "PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond." in: Trends in biochemical sciences, Vol. 22, Issue 9, pp. 355-8, (1997) (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, (1991) (PubMed).

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4 (PAK4 Products)

Synonyms: 5730488L07Rik antibody, AW555722 antibody, mKIAA1142 antibody, im:5916170 antibody, wu:fd12b01 antibody, zgc:92014 antibody, p21 (RAC1) activated kinase 4 antibody, p21 protein (Cdc42/Rac)-activated kinase 4 antibody, PAK4 antibody, Pak4 antibody, pak4 antibody

Background: Name/Gene ID: PAK4
Subfamily: PAK (STE20)
Family: Protein Kinase

Synonyms: PAK4, KIAA1142, p21(CDKN1A)-activated kinase 4, PAK-4, p21-activated kinase 4

Gene ID: 10298

NCBI Accession: NP_001099708

UniProt: O96013

Pathways: RTK Signaling