NFKB2 antibody (pSer869)

Catalog No. ABIN318077

Product Details anti-NFKB2 Antibody

Target: NFKB2 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2))

Binding Specificity: pSer869

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of NFκB-p100/p52 pSer869 protein. (region surrounding Ser869)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunoprecipitation: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NFKB2

Target: NFKB2 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2))

Alternative Name: NFKB2 / NF-kappa-B p100/p52 (NFKB2 Products)

Synonyms: NF-kappaB2 antibody, lyt antibody, p49 antibody, p49/p100 antibody, p50B antibody, p52 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100 antibody, Nfkb2 antibody

Background: The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.Synonyms: DNA-binding factor KBF2, H2TF1, KBF2, LYT10, Lymphocyte translocation chromosome 10, NF kappa B, NF-kB p100, NF-kB p52, Nuclear factor NF-kappa-B p100 subunit, Oncogene Lyt-10

Molecular Weight: approx. 100, 120 kDa

Gene ID: 4791

NCBI Accession: NP_001070961

UniProt: Q00653

Pathways: Toll-Like Receptors Cascades