HA-Tag antibody

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Antigen
Reactivity
Tag
445
2
1
1
1
1
1
Host
Rabbit
177
175
40
34
20
3
Clonality
Polyclonal
Conjugate
Un-conjugated
43
29
23
8
8
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
355
198
113
109
79
67
53
39
15
13
10
7
7
5
4
4
4
4
2
2
2
1
1
1
1
1
1
Options
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Immunogen Keyhole limpet hemocyanin conjugated epitope tag peptide (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
Sequence YPYDVPDYA
Isotype IgG
Purification Purified
Alternative Name HA Epitope Tag (YPYDVPDYA)
Target Type Tag
Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Application Notes Immunohistochmemistry on paraffin sections (Requires antigen retrieval using heattreatment prior to staining of paraffin embedded sections. Citrate buffer is recommendedfor this purpose). ELISA. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 0.02 M Potassium phosphate, 0.12 M Sodium chloride pH 7.2, 0.01 % Sodium Azide (NaN3)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Background publications Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

Wilson, Niman, Houghten, Cherenson, Connolly, Lerner: "The structure of an antigenic determinant in a protein." in: Cell, Vol. 37, Issue 3, pp. 767-78, 1984 (PubMed).