UNG antibody (N-Term)

Catalog No. ABIN318787

This Rabbit Polyclonal antibody specifically detects UNG in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for UNG antibody (N-Term) (ABIN318787)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNG antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UNG Antibody

Binding Specificity: N-Term

Specificity: This antibody recognises uracil-DNA glycosylase 1 (UNG1), a member of the uracil-DNA glycosylase and cyclin family.

Purification: Purified

Immunogen: 14 amino acid peptide sequence near the amino terminus of human UNG1.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 - 1.0g/mL, detects a band of approximately 34.6 kDa in C2C/2 celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for UNG

Target: UNG (Uracil-DNA Glycosylase (UNG))

Background: Two isoforms of UNG exist, isoform 1 is expressed in the mitochondria of in skeletal muscle, heart and testicles while isoform 2 (UNG2) is more abundant in proliferating tissues and expressed in the nucleus. Uracil residues can be misincorporated into DNA by DNA polymerase or by deamination of cytosine. UNG1 is a major enzyme in base excision repair, excising the misincorporated uracil residues in a process crucial to increasing immunoglobulin diversity. In association with activation-induced cytosine deaminase, UNG is also essential to the generation of strand breaks that initiate class switch recombination. UNG is also involved in the innate immune response against retroviral infections, HIV-1 accessory protein Vpr induces the rapid degradation of UNG2.Synonyms: DGU, UDG, UNG1, UNG15

Gene ID: 7374

NCBI Accession: NP_003353

UniProt: P13051

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response