PPARG antibody (Isoform 1)

Catalog No. ABIN318834

The Rabbit Polyclonal anti-PPARG antibody (ABIN318834) specifically detects PPARG in EIA. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Hamster, Monkey, Duck and Mink samples.

Quick Overview for PPARG antibody (Isoform 1) (ABIN318834)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Hamster, Monkey, Duck, Mink

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-PPARG Antibody

Binding Specificity: AA 255-268, Isoform 1

Specificity: This antibody detects human peroxisome proliferator-activated receptor gamma (PPARG).

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 255 - 268 of human PPAR gamma isoform 1.

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 8000 - 1: 32000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.01 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPAR-gamma

Background: PPARG is a member of the nuclear hormone receptor subfamily of transcription factors. Upon activation, PPARG forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. PPARG is principally involved in the cellular assimilation of lipids, with key roles in adipocyte differentiation and adipogenesis. Two alternatively spliced isoforms of PPARG exist, termed PPARG1 and PPARG2, which are identical except for an additional 30 amino acids at the N-terminus of PPARG2. This results in a predominant adipocyte localisation for PPARG2, whilst PPARG1 is ubiquitously expressed. PPARG has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer.Synonyms: NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma

Gene ID: 5468

NCBI Accession: NP_005028

UniProt: P37231

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation