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APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 12-28)

Details for Product anti-APAF1 Antibody No. ABIN319002, Supplier: Log in to see
Antigen
Alternatives
anti-Human APAF1 antibody for Western Blotting
Epitope
AA 12-28
46
44
17
13
13
8
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
190
104
85
4
2
2
2
1
1
Host
Rabbit
164
23
13
3
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
149
60
60
42
29
22
15
9
8
5
4
Supplier
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Immunogen Synthetic peptide corresponding to amino acids 12-28 of Human Apaf1
Isotype IgG
Specificity This antibody recognises a 130 kDa band in Western blots.
Purification Afinity Chromatography
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Apaf1 (Apoptosis protease activating factor 1) is the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8.Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
NCBI Accession NP_001151
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot: 1/1000 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 12-28) (ABIN319002) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody
 image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 12-28) (ABIN319002) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody (Image 2)
Background publications Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).