V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) (pTyr1328) antibody

Antigen: V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3)

Synonyms: ERBB3

Epitope: pTyr1328

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN319274

Additional Information

Characteristics: Synonyms: ERBB-3, c-erbB-3, HER-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cellsurface receptor HER3, Proto-oncogene-like protein c-ErbB-3

Alternative name: ERBB3 / HER3

Swiss-Prot: P21860

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from humanHer3/ErbB3 around the phosphorylation site of Tyrosine 1328 (P-D-YP-W-H).

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family ofreceptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulinbinding domain but not an active kinase domain. It can therefore bind this ligand butcannot convey a signal into the cell via protein phosphorylation. However it does formheterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation ordifferentiation. Amplification of this gene and/or overexpression of its protein have beenreported in numerous cancers including prostate, bladder and breast tumors. Alternatetranscriptional splice variants encoding different isoforms have been characterized. Oneisoform lacks the intermembrane region and is secreted outside the cell. This form acts tomodulate the activity of the membrane-bound form. Additional splice variants have alsobeen reported but they have not been thoroughly characterized.

Specificity: Antibody AP08032PU detects endogenous levels of Her3/ErbB3 only when phosphorylatedat Tyrosine 1328. Species: Human, Mouse and Rat.

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azideand 50% Glycerol.

Storage: Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only