CDK6 antibody (pTyr24)

Catalog No. ABIN319289

There are 2+ publications for this product available. The Rabbit Polyclonal anti-CDK6 antibody is suitable to detect CDK6 in samples from Human and Mouse. It has been validated for WB, IF and IHC (p).

Quick Overview for CDK6 antibody (pTyr24) (ABIN319289)

Target: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDK6 Antibody

Binding Specificity: pTyr24

Specificity: Antibody AP08040PU detects endogenous levels of CDK6 only when phosphorylated at Tyrosine 24.

Purification: Affinity Chromatography

Immunogen: Synthetic phosphopeptide derived from human CDK6 around the phosphorylation site of Tyrosine 24 (G-A-YP-G-K).

Application Details

Application Notes: Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

References for anti-CDK6 antibody (ABIN319289)

Bychkov, Shulepko, Vasileva, Sudarikova, Kirpichnikov, Lyukmanova: "ASIC1a Inhibitor mambalgin-2 Suppresses the Growth of Leukemia Cells by Cell Cycle Arrest." in: Acta naturae, Vol. 12, Issue 2, pp. 101-116, (2020) (PubMed).

Bychkov, Shulepko, Osmakov, Andreev, Sudarikova, Vasileva, Pavlyukov, Latyshev, Potapov, Kirpichnikov, Shenkarev, Lyukmanova: "Mambalgin-2 Induces Cell Cycle Arrest and Apoptosis in Glioma Cells via Interaction with ASIC1a." in: Cancers, Vol. 12, Issue 7, (2020) (PubMed).

Target Details for CDK6

Target: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Alternative Name: CDK6

Background: Cdk6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.Synonyms: Cell division protein kinase 6, PLSTIRE, Serine/threonine-protein kinase PLSTIRE

Gene ID: 1021

NCBI Accession: NP_001138778

UniProt: Q00534

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases