Spleen tyrosine Kinase (SYK) (AA 321-325) antibody

Details for Product No. ABIN319415
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Antigen
Synonyms p72-Syk, Sykb, p72syk
Epitope
AA 321-325
(40), (28), (23), (19), (12), (10), (8), (8), (7), (7), (7), (7), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(259), (110), (103), (37), (27), (25), (12), (12), (3), (1)
Host
Rabbit
(206), (52), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(207), (99), (92), (71), (48), (39), (30), (23), (3), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN319415
308.00 $
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Immunogen Peptide sequenece around amino acids 321~325 (N-P-Y-E-P) derived from Human Syk.
Specificity This antibody detects endogenous levels of total Syk protein.
Purification Immunoaffinity Chromatography using epitope-specific immunogen.
Alternative Name SYK
Background Syk (72 kDa) is a non receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.
Alternate names: Spleen tyrosine kinase, Tyrosine-protein kinase SYK
Gene ID 6850
NCBI Accession NP_001128524
UniProt P43405
Research Area Signaling
Application Notes Western Blot: 1/500~1/1000. Immunofluorescence: 1/100~1/200. Immunohistochemistry on Paraffin Sections: 1/50~1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20°C.
Expiry Date 12 months
Supplier Images
anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody
anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (2) anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (Image 2)
anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (3) anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (Image 3)
Background publications Turner, Schweighoffer, Colucci et al.: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000 (PubMed).

Zhang, Billingsley, Kincaid et al.: "Phosphorylation of Syk activation loop tyrosines is essential for Syk function. An in vivo study using a specific anti-Syk activation loop phosphotyrosine antibody." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35442-7, 2000 (PubMed).

Validation Images
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