Selectin E/CD62e antibody (FITC)

Catalog No. ABIN319450

Product Details anti-Selectin E/CD62e Antibody (FITC)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognizes the CD62E and CD62P cell surface antigens. Although previously thought to recognise only Human CD62E, recent data shows that this antibody also recognizes Human CD62P, binding to a common epitope shared by these members of the selectin family.

Cross-Reactivity (Details): Also reacts to: CD62P

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Immunogen: Human E-Selectin (ELAM-1). Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Clone: 1-2B6

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10l of the suggested working dilution to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: CD62E / E-Selectin (SELE Products)

Synonyms: SELE antibody, CD62E antibody, ELAM antibody, ELAM1 antibody, ESEL antibody, LECAM2 antibody, E-selectin antibody, Elam antibody, selectin E antibody, selectin, endothelial cell antibody, SELE antibody, Sele antibody

Background: CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

Gene ID: 6401

NCBI Accession: NP_000441

UniProt: P16109

Pathways: Thromboxane A2 Receptor Signaling