ATP6V0D2 antibody (Isoform 1)
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- Target See all ATP6V0D2 Antibodies
- ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))
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Binding Specificity
- Isoform 1
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Reactivity
- Tomato, Arabidopsis thaliana, Zea mays, Pteris vittata (Chinese ladder brake), Oryza sativa, Lilium longiflorum (Trumpet lily), Chlamydomonas reinhardtii, Barley
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATP6V0D2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Cross-Reactivity (Details)
- No cross-reactivity with: mangrove plants,
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 26 / 31 kDa (Arabidopsis thaliana)
- Purification
- serum
- Immunogen
- KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
- Top Product
- Discover our top product ATP6V0D2 Primary Antibody
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- Application Notes
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Recommended Dilution: 1 : 2 000 - 1 : 5000 with alkaline phosphatase (WB), 1 : 50 (IHC).
Cellular [compartment marker] of tonoplast membrane - Comment
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V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 10 µL of sterile water.
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
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Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana." in: Proteomics, Vol. 14, Issue 19, pp. 2141-55, (2014) (PubMed).
: "Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein." in: Plant cell reports, (2010) (PubMed).
: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, (2009) (PubMed).
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Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana." in: Proteomics, Vol. 14, Issue 19, pp. 2141-55, (2014) (PubMed).
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- Target
- ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))
- Alternative Name
- V-ATPase (ATP6V0D2 Products)
- Synonyms
- ATP6D2 antibody, VMA6 antibody, AI324824 antibody, V-ATPase antibody, 1620401A02Rik antibody, ATPase H+ transporting V0 subunit d2 antibody, ATPase, H+ transporting, lysosomal V0 subunit D2 antibody, ATPase H+ transporting V0 subunit D2 antibody, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 L homeolog antibody, ATP6V0D2 antibody, Atp6v0d2 antibody, atp6v0d2.L antibody
- Background
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AGI Code: At4g11150
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448 - Molecular Weight
- expected: 26 kDa, apparent: 31 kDa (Arabidopsis thaliana)
- UniProt
- Q39258
- Pathways
- Transition Metal Ion Homeostasis, Proton Transport
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