ATP6V0D2 antibody (Middle Region)

Catalog No. ABIN2786234

Product Details anti-ATP6V0D2 Antibody

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Rabbit, Guinea Pig, Dog, Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0D2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GLRLLAQAED FDQMKNVADH YGVYKPLFEA VGGSGGKTLE DVFYEREVQM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ATP6V0D2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATP6V0D2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 350 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATP6V0D2 antibody (ABIN2786234)

Hamamura, Chen, Nishimura, Tanjung, Sudo, Yokota: "Predicting and validating the pathway of Wnt3a-driven suppression of osteoclastogenesis." in: Cellular signalling, Vol. 26, Issue 11, pp. 2358-69, (2014) (PubMed).

Okayasu, Nakayachi, Hayashida, Ito, Kaneda, Masuhara, Suda, Sato, Hakeda: "Low-density lipoprotein receptor deficiency causes impaired osteoclastogenesis and increased bone mass in mice because of defect in osteoclastic cell-cell fusion." in: The Journal of biological chemistry, Vol. 287, Issue 23, pp. 19229-41, (2012) (PubMed).

Target Details for ATP6V0D2

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Alternative Name: ATP6V0D2 (ATP6V0D2 Products)

Synonyms: ATP6D2 antibody, VMA6 antibody, AI324824 antibody, V-ATPase antibody, 1620401A02Rik antibody, ATPase H+ transporting V0 subunit d2 antibody, ATPase, H+ transporting, lysosomal V0 subunit D2 antibody, ATPase H+ transporting V0 subunit D2 antibody, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 L homeolog antibody, ATP6V0D2 antibody, Atp6v0d2 antibody, atp6v0d2.L antibody

Background: ATP6V0D2 is the subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. ATP6V0D2 may play a role in coupling of proton transport and ATP hydrolysis.
Alias Symbols: ATP6D2, FLJ38708, VMA6
Protein Interaction Partner: AGO3, RBM15B, RPS3, NDUFB8, ADRM1,
Protein Size: 350

Molecular Weight: 40 kDa

Gene ID: 245972

NCBI Accession: NM_152565, NP_689778

UniProt: Q8N8Y2

Pathways: Transition Metal Ion Homeostasis, Proton Transport