ATP6V0D2 antibody (Middle Region)

Catalog No. ABIN2786234

This Rabbit Polyclonal antibody specifically detects ATP6V0D2 in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Rabbit, Guinea Pig, Cow, Horse and Pig. It has been mentioned in 2+ publications

Quick Overview for ATP6V0D2 antibody (Middle Region) (ABIN2786234)

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Guinea Pig, Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0D2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V0D2 Antibody

Binding Specificity: Middle Region

Sequence: GLRLLAQAED FDQMKNVADH YGVYKPLFEA VGGSGGKTLE DVFYEREVQM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ATP6V0D2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATP6V0D2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 350 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATP6V0D2 antibody (ABIN2786234)

Hamamura, Chen, Nishimura, Tanjung, Sudo, Yokota: "Predicting and validating the pathway of Wnt3a-driven suppression of osteoclastogenesis." in: Cellular signalling, Vol. 26, Issue 11, pp. 2358-69, (2014) (PubMed).

Okayasu, Nakayachi, Hayashida, Ito, Kaneda, Masuhara, Suda, Sato, Hakeda: "Low-density lipoprotein receptor deficiency causes impaired osteoclastogenesis and increased bone mass in mice because of defect in osteoclastic cell-cell fusion." in: The Journal of biological chemistry, Vol. 287, Issue 23, pp. 19229-41, (2012) (PubMed).

Target Details for ATP6V0D2

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Alternative Name: ATP6V0D2

Background: ATP6V0D2 is the subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. ATP6V0D2 may play a role in coupling of proton transport and ATP hydrolysis.
Alias Symbols: ATP6D2, FLJ38708, VMA6
Protein Interaction Partner: AGO3, RBM15B, RPS3, NDUFB8, ADRM1,
Protein Size: 350

Molecular Weight: 40 kDa

Gene ID: 245972

NCBI Accession: NM_152565, NP_689778

UniProt: Q8N8Y2

Pathways: Transition Metal Ion Homeostasis, Proton Transport