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Keratin 14 (KRT14) (C-Term) antibody

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Synonyms Krt14, KRT14, CK14, ouro2, EBS3, EBS4, K14, NFJ, AI626930, Krt-1.14, Krt1-14, Ka14
(87), (7), (6), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(207), (50), (47), (11), (9), (4), (3), (3), (3)
(112), (95), (5)
Clonality (Clone)
Monoclonal ()
(9), (3), (2)
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(127), (83), (71), (53), (52), (44), (32), (15), (4), (2), (2), (2), (1)
Pubmed 10 references available
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Quantity 20 μg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Last 15 C-terminal residues of Human Cytokeratin 14 conjugated to Thyroglobulin.
Clone LL002
Isotype IgG3
Specificity This antibody recognises Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14.
Purification Ion Exchange Chromatography
Alternative Name Cytokeratin 14 (KRT14 Antibody Abstract)
Background Cytokeratin is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14
Gene ID 3861
NCBI Accession NP_000517
UniProt P02533
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes Flow Cytometry: Use 10 μL of 1/100 diluted antibody to label 10^6 cells in 100 μL (Membrane permeabilisation is required). Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/200): This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer is recommended for this purpose, Enzyme digestion is not recommended. Recommended Positive Control: Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Abdeen, Salah, Maly et al.: "Wwox inactivation enhances mammary tumorigenesis." in: Oncogene, Vol. 30, Issue 36, pp. 3900-6, 2011 (PubMed).

Turner, Subramanian, Thomas et al.: "Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty." in: European urology, Vol. 59, Issue 3, pp. 447-54, 2011 (PubMed).

Takahashi, Yoshida, Komine et al.: "Newly established cell lines from mouse oral epithelium regenerate teeth when combined with dental mesenchyme." in: In vitro cellular & developmental biology. Animal, Vol. 46, Issue 5, pp. 457-68, 2010 (PubMed).

Matos, Faustino, Lopes et al.: "Detection of lymph node micrometastases in malignant mammary tumours in dogs by cytokeratin immunostaining." in: The Veterinary record, Vol. 158, Issue 18, pp. 626-30, 2006 (PubMed).

Varley, Stahlschmidt, Smith et al.: "Activation of peroxisome proliferator-activated receptor-gamma reverses squamous metaplasia and induces transitional differentiation in normal human urothelial cells." in: The American journal of pathology, Vol. 164, Issue 5, pp. 1789-98, 2004 (PubMed).

Hale, Markert: "Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 1, pp. 617-24, 2003 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Wetzels, Holland, van Haelst et al.: "Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast." in: The American journal of pathology, Vol. 134, Issue 3, pp. 571-9, 1989 (PubMed).

Lane, Alexander: "Use of keratin antibodies in tumor diagnosis." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 165-79, 1991 (PubMed).

Purkis, Steel, Mackenzie et al.: "Antibody markers of basal cells in complex epithelia." in: Journal of cell science, Vol. 97 ( Pt 1), pp. 39-50, 1991 (PubMed).

Catalog No. ABIN319880
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