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V5 Epitope Tag antibody

WB, IP, IHC (fro), RIA, EIA Host: Mouse Monoclonal SV5-Pk1 unconjugated
Catalog No. ABIN319983
  • Target See all V5 Epitope Tag products
    V5 Epitope Tag
    Reactivity
    Please inquire
    Host
    • 60
    • 41
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    Mouse
    Clonality
    • 57
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    Monoclonal
    Conjugate
    • 66
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    This V5 Epitope Tag antibody is un-conjugated
    Application
    • 101
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    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)
    Sequence
    GKPIPNPLLG LDST
    Purification
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    Immunogen
    Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
    Clone
    SV5-Pk1
    Isotype
    IgG2a
  • Application Notes
    ELISA (1/1000-1/5000). Western Blot (1/1000-1/5000). Immunoprecipitation. Radioimmunoassays. Immunohistochemistry on Frozen Sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is manufactued under an exclusive license from the University of St. Andrews,UK.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    V5 Epitope Tag
    Alternative Name
    Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag Products)
    Target Type
    Tag
    Background
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
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