V5 Epitope Tag antibody

Details for Product No. ABIN319983, Supplier: Log in to see
Antigen
Reactivity
Tag
404
3
3
3
3
Host
Mouse
220
135
37
9
5
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
29
27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
342
266
123
91
70
65
53
36
27
13
8
8
5
4
4
2
2
2
1
1
1
1
Options
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Immunogen Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Sequence GKPIPNPLLG LDST
Clone SV5-Pk1
Isotype IgG2a
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Target Type Tag
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Research Area Tags/Labels
Application Notes ELISA (1/1000-1/5000). Western Blot (1/1000-1/5000). Immunoprecipitation. Radioimmunoassays. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is manufactued under an exclusive license from the University of St. Andrews,UK.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Zhang, Yu, Liebeskind: "N-amidation by copper-mediated cross-coupling of organostannanes or boronic acids with o-acetyl hydroxamic acids." in: Organic letters, Vol. 10, Issue 14, pp. 3005-8, 2008 (PubMed).

Sanchez Garcia, Ciufo, Yang, Kearsey, MacNeill: "The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit." in: Nucleic acids research, Vol. 32, Issue 10, pp. 3005-16, 2004 (PubMed).

Young, Chatziandreou, He, Goodbourn, Lamb, Randall: "Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells." in: Journal of virology, Vol. 75, Issue 7, pp. 3363-70, 2001 (PubMed).

Dunn, ODowd, Randall: "Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 141-50, 1999 (PubMed).

Hanke, Young, Doyle, Jones, Randall: "Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding." in: Journal of virological methods, Vol. 53, Issue 1, pp. 149-56, 1995 (PubMed).

Jaffray, Wood, Hay: "Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65." in: Molecular and cellular biology, Vol. 15, Issue 4, pp. 2166-72, 1995 (PubMed).

Rodriguez, Michalopoulos, Arenzana-Seisdedos, Hay: "Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2413-9, 1995 (PubMed).

Arenzana-Seisdedos, Thompson, Rodriguez, Bachelerie, Thomas, Hay: "Inducible nuclear expression of newly synthesized I kappa B alpha negatively regulates DNA-binding and transcriptional activities of NF-kappa B." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2689-96, 1995 (PubMed).

Hirst, Fisher, McAndrew, Goding: "The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal." in: The EMBO journal, Vol. 13, Issue 22, pp. 5410-20, 1994 (PubMed).

Randall, Hanke, Young, Southern: "Two-tag purification of recombinant proteins for the construction of solid matrix-antibody-antigen (SMAA) complexes as vaccines." in: Vaccine, Vol. 11, Issue 12, pp. 1247-52, 1994 (PubMed).

Randall, Young, Hanke, Szawlowski, Botting: "Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming." in: Vaccine, Vol. 12, Issue 4, pp. 351-8, 1994 (PubMed).

Hanke, Szawlowski, Randall: "Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen." in: The Journal of general virology, Vol. 73 ( Pt 3), pp. 653-60, 1992 (PubMed).

Southern, Young, Heaney, Baumgärtner, Randall: "Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics." in: The Journal of general virology, Vol. 72 ( Pt 7), pp. 1551-7, 1991 (PubMed).