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CD8 antibody (CD8a Molecule) (FITC)

Details for Product anti-CD8A Antibody No. ABIN320012, Supplier: Log in to see
Antigen
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Alternatives
anti-Human CD8 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human, Monkey
1058
532
167
68
47
40
25
22
20
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19
16
15
13
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12
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11
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10
8
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3
3
2
2
2
2
1
1
1
Host
Mouse
1285
512
206
Clonality (Clone)
Monoclonal ()
Conjugate
This CD8 antibody is conjugated to FITC
258
217
142
86
52
42
40
39
30
30
29
28
27
26
25
25
25
25
25
21
21
18
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17
11
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10
8
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3
3
3
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3
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3
2
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2
2
2
2
1
1
1
1
1
1
1
1
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1
1
1
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1
1
1
1
1
Application
Flow Cytometry (FACS)
1617
452
356
343
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238
214
37
33
25
23
17
14
10
7
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5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Immunogen Normal Human peripheral blood lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1
Specificity This antibody recognises the CD8 cell surface glycoprotein.
Purification Ion Exchange Chromatography.
Alternative Name CD8 (CD8A Antibody Abstract)
Background CD8 is expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells. The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Flow cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells orcells or 100l human whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Nelson, Lever, Dean, Savage, Salguero, Pearce, Stevens, Simpson: "Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus)." in: Journal of medical microbiology, Vol. 59, Issue Pt 9, pp. 1107-13, 2010 (PubMed).

Kap, van Meurs, van Driel, Koopman, Melief, Brok, Laman, t Hart: "A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 12, pp. 1159-67, 2009 (PubMed).

Gibbings, Marcet-Palacios, Sekar, Ng, Befus: "CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses." in: BMC immunology, Vol. 8, pp. 12, 2007 (PubMed).

Zarkesh-Esfahani, Pockley, Metcalfe, Bidlingmaier, Wu, Ajami, Weetman, Strasburger, Ross: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989 (PubMed).