CDC42 antibody (AA 38-87)

Catalog No. ABIN6739077

The Rabbit Polyclonal anti-CDC42 antibody is suitable to detect CDC42 in samples from Human, Mouse, Rat, Cow, Dog, Pig, Monkey, Chicken, Rabbit, Horse, Xenopus laevis, Insect, Guinea Pig and Avian. It has been validated for WB.

Quick Overview for CDC42 antibody (AA 38-87) (ABIN6739077)

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Monkey, Chicken, Rabbit, Horse, Xenopus laevis, Insect, Guinea Pig, Avian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC42 Antibody

Binding Specificity: AA 38-87

Specificity: Human CDC42

Predicted Reactivity: Percent identity by BLAST analysis: Monkey, Dog, Rabbit, Chicken, Xenopus, Catfish, Pufferfish, Eye worm, Seq squirt, Mosquito, Nematode, Tunicate, Trichomonas (100%).

Purification: Immunoaffinity purified

Immunogen: ynthetic peptide located between aa38-87 of human CDC42 (P60953, NP_001782). Percent identity by BLAST analysis: Human, Chimpanzee, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Sablefish, Mosquito, Tunicate, California sea hare, Red sea bream, African clawed frog, Crab-eating macaque, Channel catfish, blue catfish, Sponge, Green puffer, Common rockweed, Dumeril's clam worm, Croceine croaker, Choanoflagellate, Japanese quail, Brown alga (100%).

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2 % sucrose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CDC42

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Alternative Name: CDC42

Background: Name/Gene ID: CDC42

Synonyms: CDC42, G25K, G25K GTP-binding protein, Growth-regulating protein, CDC42Hs, GTP-binding protein, 25kD

Gene ID: 998

NCBI Accession: NP_001782

UniProt: P60953

Pathways: MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling