DRD5 antibody (Internal Region)

Catalog No. ABIN334388

The Goat Polyclonal anti-DRD5 antibody has been validated for ELISA, IF and FACS. It is suitable to detect DRD5 in samples from Human.

Quick Overview for DRD5 antibody (Internal Region) (ABIN334388)

Target: DRD5 (Dopamine Receptor D5 (DRD5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DRD5 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-DRD5 Antibody

Binding Specificity: Internal Region

Purpose: DRD5

Sequence: NREVDNDEEE GPFD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NREVDNDEEEGPFD, from the internal region of the protein sequence according to NP_000789.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 55 kDa in Caco-2 and U2OS cell lysate after 1-2 μg/mL antibody staining (calculated MW of 53.0 kDa according to NP_000789.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membranes of U251 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recomm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DRD5

Target: DRD5 (Dopamine Receptor D5 (DRD5))

Alternative Name: DRD5

Background: DRD5, dopamine receptor D5 , DBDR, DRD1B, DRD1L2, MGC10601 , D1beta dopamine receptor, dopamine receptor D1B

Gene ID: 1816

NCBI Accession: NP_000789

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity