DRD5 antibody (2nd Extracellular Loop)

Catalog No. ABIN7886287

The Rabbit Polyclonal anti-DRD5 antibody is suitable to detect DRD5 in samples from Rat. It has been validated for WB, IHC, FACS, IF and LCI.

Quick Overview for DRD5 antibody (2nd Extracellular Loop) (ABIN7886287)

Target: DRD5 (Dopamine Receptor D5 (DRD5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-DRD5 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 199-211

Purpose: A Rabbit Polyclonal Antibody to D5 Dopamine Receptor

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Unlikely to recognize human samples.

Predicted Reactivity: Mouse - 12,13 amino acid residues identical

Characteristics: Anti-D5 Dopamine Receptor (extracellular) Antibody is directed against an epitope located at the 2nd extracellular loop of the rat D5 dopamine receptor. Anti-D5 Dopamine Receptor (extracellular) Antibody (ABIN7043111, ABIN7044234 and ABIN7044235) can be used in western blot analysis and immunohistochemical applications, and recognizes DRD5 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: EEGWELEGRTENC, corresponding to amino acid residues 199-211 of rat DRD5 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235250)

Blocking Peptide: (ABIN7235250)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for DRD5

Target: DRD5 (Dopamine Receptor D5 (DRD5))

Alternative Name: DRD5

Background:

Synonyms: DRD5, D(1B) dopamine receptor

Description: The D5 dopamine receptor (D5 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and Schizophrenia.1All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1 The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1The D5 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex, hippocampus and striatum. In the periphery, the D5 receptor has been localized in the adrenal cortex, kidney and intestinal tract.The exact physiological function of the D5 receptor subtype remains poorly understood. Studies with D5 receptor knockout mice have shown no overt alterations in locomotor or cognitive functions. However, knockout mice do develop severe hypertension suggesting a role of the D5 receptor in the modulation of neuronal pathways regulating blood pressure responses.2,3

Gene ID: 25195

NCBI Accession: NM_000798

UniProt: P25115

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity