gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3) antibody

Antigen: gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3)

Synonyms: Cp1, beta3, Gabrb-3, AW049585, A230092K12Rik, GABRB3, gabrb3, MGC122298, DKFZp469F2217, LOC100230475

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334404

Additional Information

Alternative name: GABRB3

Gene ID: 2562, 14402 (mouse), 24922 (rat)

Immunogen: C-EKTAKAKNDRSK

Description: All names and symbols: GABRB3, gamma-aminobutyric acid (GABA) A receptor, beta 3, MGC9051, GABA(A) receptor beta-3 subunit, GABA-A receptor beta-3 subunit, GABA-alpha receptor beta-2 subunit, GABAA receptor beta-3 subunit. Official Symbol: GABRB3. RefSeq number(s): NP_000805.1, NP_068712.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_000805.1, NP_068712.1). Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Tanaka, Olsen, Medina et al.: "Hyperglycosylation and reduced GABA currents of mutated GABRB3 polypeptide in remitting childhood absence epilepsy." in: American journal of human genetics, Vol. 82, Issue 6, pp. 1249-61, 2008 (PubMed).