Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (Internal Region) antibody

Details for Product No. ABIN334405
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Antigen
Synonyms CPSQ1, GAD, SCP, GAD67, GAD-67, GAD1, GLUTAMATE DECARBOXYLASE, GLUTAMATE DECARBOXYLASE 1, MKP11.30, MKP11_30, glutamate decarboxylase, cpsq1, gad1, gad1-A, gad67, GAD2, AO090005000539, AO090103000342
Epitope
Internal Region
(13), (7), (6), (6), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(91), (52), (49), (7), (5), (5), (5), (1), (1)
Host
Goat
(76), (16), (8), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(80), (33), (31), (26), (10), (8), (7), (5), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN334405
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.
Specificity This antibody is expected to recognize isoform GAD67. There is
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Pig (Porcine)
Cross-Reactivity (Details) No cross-reactivity expected with GAD2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GAD1 (GAD67)
Background Synonyms: GAD1, glutamate decarboxylase 1 (brain, 67kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25
Gene ID 2571, 14415, 24379
NCBI Accession NP_000808.2
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 65 kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 66.9 kDa according to NP_000808.2). Recommended concentration: 0.5-1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (Internal Region) antibody ABIN334405 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Huang, Akbarian: "GAD1 mRNA expression and DNA methylation in prefrontal cortex of subjects with schizophrenia." in: PLoS ONE, Vol. 2, Issue 8, pp. e809, 2007 (PubMed).

Hosts (76), (16), (8), (4)
Reactivities (91), (52), (49), (7), (5), (5), (5), (1), (1)
Applications (80), (33), (31), (26), (10), (8), (7), (5), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (7), (6), (6), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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