Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G) (AA 18-28) antibody

Antigen: Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G)

Synonyms: CR6, DDIT2, GRP17, GADD45gamma, PRG6, CRIF1, CKBBP2, Plinp1, MGC4667, MGC4758, PLINP-1, MGC77806, GADD45G, cr6, ddit2, grp17, MGC89481, xgadd45g, gadd45gamma, XGadd45-gamma, MGC137546, OIG37, C86281, AI327420

Epitope: AA 18-28

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334406

Additional Information

Alternative name: GADD45gamma (aa 18 to 28)

Gene ID: 10912, 23882 (mouse), 291005 (rat)

Immunogen: C-RMQGAGKALHE

Description: All names and symbols: growth arrest and DNA-damage-inducible, gamma, CR6, DDIT2, GADD45gamma, GRP17, GADD45-gamma, gadd-related protein, 17 kD, growth arrest and DNA-damage-inducible gamma. Official Symbol: GADD45G. RefSeq number(s): NP_006696.1

Specificity: Human, Mouse, Rat

Synonyms: CR6, DDIT2, GRP17, GADD45gamma, PRG6, CRIF1, CKBBP2, Plinp1, MGC4667, MGC4758, PLINP-1, MGC77806, GADD45G, cr6, ddit2, grp17, MGC89481, xgadd45g, gadd45gamma, XGadd45-gamma, MGC137546, OIG37, C86281, AI327420

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 23kDa band in Human Testis and Thyroid Gland lysates and in lysates of cell line A549 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_006696.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Zerbini, Wang, Czibere et al.: "NF-kappa B-mediated repression of growth arrest- and DNA-damage-inducible proteins 45alpha and gamma is essential for cancer cell survival." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 37, pp. 13618-23, 2004 (PubMed).