Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1) antibody

Antigen: Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1)

Synonyms: EAA3, EEA3, GLR5, GLUR5, GluR5, GRIK1, MGC142923, MGC148595, grik1, fv36b03, wu:fv36b03

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334410

Additional Information

Alternative name: GLuR5 / GRIK1

Gene ID: 2897, 14805 (mouse), 29559 (rat)

Immunogen: QCKQTHPTNSTS

Description: All names and symbols: GLUR5, GRIK1, glutamate receptor, ionotropic, kainate 1, EAA3 , EEA3 , GLR5 , excitatory amino acid receptor 3. Official Symbol: GRIK1. RefSeq number(s): NP_000821.1, NP_783300.1

Specificity: This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1. Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al , J Neurosci. 2004 Oct 13,24(41):8986-93., PMID: 15483117). Recommended concentration: 1-3µg/ml. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334410 (1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Martin, Nishimune, Mellor et al.: "SUMOylation regulates kainate-receptor-mediated synaptic transmission." in: Nature, Vol. 447, Issue 7142, pp. 321-5, 2007 (PubMed).