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ITCH antibody (Internal Region)

This Goat Polyclonal antibody specifically detects ITCH in ELISA, WB, IF and FACS. It exhibits reactivity toward Human.
Catalog No. ABIN334426

Quick Overview for ITCH antibody (Internal Region) (ABIN334426)

Target

See all ITCH Antibodies
ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity

  • 45
  • 35
  • 13
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 48
  • 4
Goat

Clonality

  • 44
  • 8
Polyclonal

Conjugate

  • 35
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ITCH antibody is un-conjugated

Application

  • 36
  • 32
  • 12
  • 5
  • 3
  • 3
  • 1
  • 1
ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade

Verified
  • Binding Specificity

    • 11
    • 8
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Purpose

    ITCH / AIF4

    Sequence

    EIKSHDLKPN GGN

    Cross-Reactivity

    Cow, Dog, Human, Mouse, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.

    Isotype

    IgG
  • Application Notes

    Western Blot: Approx 90 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4 kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

    Alternative Name

    ITCH

    Background

    ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiqui

    Gene ID

    83737, 16396, 311567

    NCBI Accession

    NP_113671, NP_001244066, NP_001244067

    Pathways

    Activation of Innate immune Response, CXCR4-mediated Signaling Events
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