ITCH antibody (Middle Region)

Catalog No. ABIN3043253

The Rabbit Polyclonal anti-ITCH antibody is suitable to detect ITCH in samples from Human, Mouse, Rat and Monkey. It has been validated for WB and FACS.

Quick Overview for ITCH antibody (Middle Region) (ABIN3043253)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ITCH Antibody

Binding Specificity: Middle Region

Purpose: Anti-ITCH/AIP4 Antibody

Sequence: AMQQFNQRFI YGNQDLFATS QSKEFDPL

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by one amino acid.

Characteristics: Anti-ITCH/AIP4 Antibody. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ITCH/AIP4, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH

Background:

Background: ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene Full Name: itchy E3 ubiquitin protein ligase

Sequence Similarities: Contains 1 C2 domain.

Molecular Weight: 103 kDa

Gene ID: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events