Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH) antibody

Antigen: Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH)

Synonyms: AIF4, AIP4, NAPP1, dJ468O1.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334426

Additional Information

Alternative name: ITCH / AIF4

Gene ID: 83737, 16396 (mouse), 311567 (rat)

Immunogen: C-EIKSHDLKPNGGN

Description: All names and symbols: ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiquitin protein ligase ITCH. Official Symbol: ITCH. RefSeq number(s): NP_113671.3

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kDa according to NP_113671.3). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334426 (0.03µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Di Marcotullio, Ferretti, Greco et al.: "Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination." in: Nature cell biology, Vol. 8, Issue 12, pp. 1415-23, 2006 (PubMed).