MutY Homolog (E. Coli) (MUTYH) antibody

Antigen: MutY Homolog (E. Coli) (MUTYH)

Synonyms: MYH, CYP2C, MGC4416, Myh, Mutyha, Mutyhb, Mutyhc, Mutyhbeta, Mutyhalpha, 5730495A01Rik, MGC156598, MUTYH, MGC128714, MGC145569

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334441

Additional Information

Alternative name: MUTYH

Gene ID: 4595

Immunogen: C-HISTDAHSLNSAAQ

Description: All names and symbols: MUTYH, MYH, mutY homolog (E. coli), mutY (E. coli) homolog. Official Symbol: MUTYH. RefSeq number(s): NP_036354.1, NP_001041636.1, NP_001041637.1, NP_001041638.1, NP_001041639.1. Gene ontology terms: DNA glycolase, mismatch repair, DNA repair, nucleus

Specificity: This antibody is expected to recognise all reported isoforms (NP_036354.1, NP_001041636.1, NP_001041637.1, NP_001041638.1, NP_001041639.1). Human 546 aa, 535 aa, 522 aa, 521 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 60kDa band observed in Human Bone Marrow lysates (calculated MW of 60.0kDa according to NP_036354.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334441 (0.1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Slupska, Baikalov, Luther et al.: "Cloning and sequencing a human homolog (hMYH) of the Escherichia coli mutY gene whose function is required for the repair of oxidative DNA damage." in: Journal of bacteriology, Vol. 178, Issue 13, pp. 3885-92, 1996 (PubMed).