HNF1 Homeobox B (HNF1B) antibody

Antigen: HNF1 Homeobox B (HNF1B)

Synonyms: FJHN, HNF2, LFB3, TCF2, HPC11, LF-B3, MODY5, VHNF1, HNF1beta, HNF1B

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334505

Additional Information

Alternative name: TCF2 / VHNF1

Gene ID: 6928, 21410 (mouse), 25640 (rat)

Immunogen: C-QAYDRQKNPSKEER

Description: All names and symbols: TCF2, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, VHNF1 , transcription factor 2. Official Symbol: TCF2. RefSeq number(s): NP_000449.1, NP_006472.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_000449.1 and NP_006472.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identit

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334505 (0.1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Terasawa, Toyota, Sagae et al.: "Epigenetic inactivation of TCF2 in ovarian cancer and various cancer cell lines." in: British journal of cancer, Vol. 94, Issue 6, pp. 914-21, 2006 (PubMed).