Keratin 7 (KRT7) antibody

Details for Product No. ABIN335326
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Antigen
Synonyms CK7, K2C7, K7, SCL, D15Wsu77e, Krt2-7, KRT7
Reactivity
Human, Mouse (Murine), Rat (Rattus), Hamster, Dog (Canine), Pig (Porcine), Cat (Feline), Zebrafish (Danio rerio)
(190), (45), (41), (7), (7), (6), (4), (3), (3), (2), (1), (1), (1)
Host
Mouse
(114), (99), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(11), (7), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(152), (97), (55), (50), (50), (43), (38), (32), (20), (13), (10), (3), (2), (1)
Pubmed 7 references available
Quantity 100 μg
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Catalog No. ABIN335326
425.04 $
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Immunogen RCK105 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cytokeratins from the human bladder carcinoma cell line T24.
Clone RCK105
Isotype IgG1
Specificity Human, mouse, rat, hamster, canine, swine and feline.
Purification Purified
Alternative Name Cytokeratin 7 / Keratin K7
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Application Notes RCK105 reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma. RCK105 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only
Storage 4 °C
Supplier Images
anti-Keratin 7 (KRT7) antibody anti-Keratin 7 (KRT7) antibody
Product cited in: Ogawa, Saeki, Igura et al.: "Complementary expression and repulsive signaling suggest that EphB2 and ephrin-B1 are possibly involved in epithelial boundary formation at the squamocolumnar junction in the rodent stomach." in: Histochemistry and cell biology, 2013 (PubMed).

General Bauwens, De Groot, Ramaekers et al.: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Schaafsma, Ramaekers, van Muijen et al.: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

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