4-HNE antibody

Antigen: 4-HNE

Clonality: Monoclonal (HNEJ-2)

Host: Mouse

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Catalog no.: ABIN335414

Additional Information

Immunogen: 4-Hydroxy-2-nonenal (4HNE) modified keyhole limpet hemocyanin.

Format: Lyophilized

Isotype: IgG1-kappa  (Matching secondary antibodies)

Clone: HNEJ-2

Specificity: Specific for 4HNE-Lysine, 4-HNE-histidine and 4-HNE cysteine adduct.By inhibition test, this antibody has a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE cysteine adduct. This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. By inhibition test, this antibody shows a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE-cysteine adduct.

Application Details

Application Notes: Immunohistochemistry [ref.1] (Recommended concentration: 25 ug/mL IgG). Western blotting [ref.2] (Recommended concentration: 15 ug/mL IgG) User should determine optimum titer for each application.

Purification: Ammonium sulphate purified IgG

Buffer: 100ug/mL IgG in 50mM Tris buffered saline (TBS).

Storage: Less than -20°C, After reconstitution, store as frozen aliquots and avoid repaeted freeze & thaw. Reconstitue with 200µl of distilled water.

Research Area: Neurology

Restrictions: For Research Use only

Images

anti-4-HNE antibody

Publications

Publications: Toyokuni, Miyake, Hiai et al.: "The monoclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct." in: FEBS letters, Vol. 359, Issue 2-3, pp. 189-91, 1995 (PubMed).

Tanaka, Nishiyama, Okada et al.: "Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 77, Issue 2, pp. 145-55, 1997 (PubMed).

Nakajima, Inokuchi, Shimazawa et al.: "Astaxanthin, a dietary carotenoid, protects retinal cells against oxidative stress in-vitro and in mice in-vivo." in: The Journal of pharmacy and pharmacology, Vol. 60, Issue 10, pp. 1365-74, 2008 (PubMed).

Sedlak, Saleh, Higginson et al.: "Bilirubin and glutathione have complementary antioxidant and cytoprotective roles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 13, pp. 5171-6, 2009 (PubMed).

Boretti, Buehler, DAgnillo et al.: "Sequestration of extracellular hemoglobin within a haptoglobin complex decreases its hypertensive and oxidative effects in dogs and guinea pigs." in: The Journal of clinical investigation, Vol. 119, Issue 8, pp. 2271-80, 2009 (PubMed).