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4-HNE antibody

Details for Product No. ABIN335414, Supplier: Login to see
Antigen
Reactivity
Chemical
1
Host
Mouse
1
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1
1
1
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Immunogen 4-Hydroxy-2-nonenal (4HNE) modified keyhole limpet hemocyanin.
Clone HNEJ-2
Isotype IgG1 kappa
Specificity Specific for 4HNE-Lysine, 4-HNE-histidine and 4-HNE cysteine adduct.By inhibition test, this antibody has a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE cysteine adduct. This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. By inhibition test, this antibody shows a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE-cysteine adduct.
Purification Ammonium sulphate purified IgG
Target Type Chemical
Research Area Neurology
Application Notes Immunohistochemistry [ref.1] (Recommended concentration: 25 ug/mL IgG). Western blotting [ref.2] (Recommended concentration: 15 ug/mL IgG) User should determine optimum titer for each application.
Restrictions For Research Use only
Format Lyophilized
Buffer 100ug/mL IgG in 50 mM Tris buffered saline (TBS).
Storage -20 °C
Supplier Images
 image for anti-4-HNE antibody (ABIN335414) anti-4-HNE antibody
Product cited in: Apidianakis, Que, Xu et al.: "Down-regulation of glutatione S-transferase α 4 (hGSTA4) in the muscle of thermally injured patients is indicative of susceptibility to bacterial infection." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 2, pp. 730-7, 2012 (PubMed).

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Ogawa, Fujii, Yoshizato et al.: "A human-type nonalcoholic steatohepatitis model with advanced fibrosis in rabbits." in: The American journal of pathology, Vol. 177, Issue 1, pp. 153-65, 2010 (PubMed).

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Kurashige, Abe, Furusu et al.: "Renoprotective effect of azelnidipine in rats." in: Biological & pharmaceutical bulletin, Vol. 31, Issue 12, pp. 2237-44, 2008 (PubMed).

Amidi, French, Chung et al.: "M-30 and 4HNE are sequestered in different aggresomes in the same hepatocytes." in: Experimental and molecular pathology, Vol. 83, Issue 3, pp. 296-300, 2007 (PubMed).

Kurimoto, Kawano, Tsuji et al.: "Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome." in: The Journal of rheumatology, Vol. 34, Issue 10, pp. 2035-43, 2007 (PubMed).

Choi, Ryu, Kim et al.: "Modulation of the purinergic P2X7 receptor attenuates lipopolysaccharide-mediated microglial activation and neuronal damage in inflamed brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 18, pp. 4957-68, 2007 (PubMed).

Kodai, Takemura, Minamiyama et al.: "S-allyl cysteine prevents CCl(4)-induced acute liver injury in rats." in: Free radical research, Vol. 41, Issue 4, pp. 489-97, 2007 (PubMed).

Tanito, Kaidzu, Anderson: "Delayed loss of cone and remaining rod photoreceptor cells due to impairment of choroidal circulation after acute light exposure in rats." in: Investigative ophthalmology & visual science, Vol. 48, Issue 4, pp. 1864-72, 2007 (PubMed).

Hirono, Yoshimoto, Suzuki et al.: "Angiotensin II receptor type 1-mediated vascular oxidative stress and proinflammatory gene expression in aldosterone-induced hypertension: the possible role of local renin-angiotensin system." in: Endocrinology, Vol. 148, Issue 4, pp. 1688-96, 2007 (PubMed).

Tanito, Elliott, Kotake et al.: "Protein modifications by 4-hydroxynonenal and 4-hydroxyhexenal in light-exposed rat retina." in: Investigative ophthalmology & visual science, Vol. 46, Issue 10, pp. 3859-68, 2005 (PubMed).

Shiraishi, Naito: "Increased expression of Leydig cell haem oxygenase-1 preserves spermatogenesis in varicocele." in: Human reproduction (Oxford, England), Vol. 20, Issue 9, pp. 2608-13, 2005 (PubMed).

Nagotani, Hayashi, Sato et al.: "Reduction of cerebral infarction in stroke-prone spontaneously hypertensive rats by statins associated with amelioration of oxidative stress." in: Stroke; a journal of cerebral circulation, Vol. 36, Issue 3, pp. 670-2, 2005 (PubMed).

Liu, Ormsby, Oja-Tebbe et al.: "Gene transfer of NAD(P)H oxidase inhibitor to the vascular adventitia attenuates medial smooth muscle hypertrophy." in: Circulation research, Vol. 95, Issue 6, pp. 587-94, 2004 (PubMed).

Yamada, Funada, Shibata et al.: "Protein-bound 4-hydroxy-2-hexenal as a marker of oxidized n-3 polyunsaturated fatty acids." in: Journal of lipid research, Vol. 45, Issue 4, pp. 626-34, 2004 (PubMed).

Tam, Gao, Leung et al.: "Androgenic regulation of oxidative stress in the rat prostate: involvement of NAD(P)H oxidases and antioxidant defense machinery during prostatic involution and regrowth." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2513-22, 2003 (PubMed).

Tanito, Nishiyama, Tanaka et al.: "Change of redox status and modulation by thiol replenishment in retinal photooxidative damage." in: Investigative ophthalmology & visual science, Vol. 43, Issue 7, pp. 2392-400, 2002 (PubMed).

Yamagami, Yamamoto, Kume et al.: "Formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: distinct localization of the two oxidatively modified products." in: Antioxidants & redox signaling, Vol. 2, Issue 1, pp. 127-36, 2001 (PubMed).

Kondo, Toyokuni, Iwasa et al.: "Persistent oxidative stress in human colorectal carcinoma, but not in adenoma." in: Free radical biology & medicine, Vol. 27, Issue 3-4, pp. 401-10, 1999 (PubMed).