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HNE antibody

Name: HNE antibody
SKU: ABIN873270
Price: 384.62 USD
Availability: InStock

HNE WB, ELISA, IF (p), IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated

The diabetes effect on IR affected cardiac lipid peroxidation in MDA content (A, n = 6), 4-HNE expression (B, n = 3), leukocyte infiltration and cardiac structure (C,D, n = 5), microcirculation (E,F, n = 10) and infarct size (G,H, n = 5) in IR CON and IR STZ rats. (HNE antibody)

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated (ABIN873270) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (HNE antibody)

IHC

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm. (HNE antibody)

ELISA

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (HNE antibody)

IHC (p)

ELISA

Mesenteric vascular Western blot graph (A) and analysis of 4-HNE (B), CaSR (C), p-eNOS (D) and BAG3 (E) are demonstrated. (HNE antibody)

6 images

(9 references)

Catalog No. ABIN873270

Datasheet as PDF

Target See all HNE Antibodies

HNE (4-Hydroxynonenal (HNE))

Reactivity

Please inquire
Host
15

14

1
Rabbit
Compatible Secondaries
Clonality
15

15
Polyclonal
Conjugate
6

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This HNE antibody is un-conjugated
Application
29

15

12

12

12

2

2

2

1
Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Monkey, Mouse, Rat

Cross-Reactivity (Details)

4-Hydroxynonenal

Purification

Purified by Protein A.

Immunogen

4 Hydroxynonenal conjugated to BSA

Isotype

IgG
anti-4-Hydroxynonenal (HNE) antibody
HNE WB, ELISA, IHC Host: Mouse Monoclonal HNEJ-2 unconjugated

anti-4-Hydroxynonenal (HNE) antibody
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 unconjugated

anti-4-Hydroxynonenal (HNE) antibody (HRP)
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 HRP

anti-4-Hydroxynonenal (HNE) antibody (FITC)
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 FITC

anti-4-Hydroxynonenal (HNE) antibody (Biotin)
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 Biotin

anti-4-Hydroxynonenal (HNE) antibody (PE)
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 PE

anti-4-Hydroxynonenal (HNE) antibody (PerCP)
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 PerCP

anti-4-Hydroxynonenal (HNE) antibody (APC)
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 APC

anti-4-Hydroxynonenal (HNE) antibody (Atto 594)
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 Atto 594

anti-4-Hydroxynonenal (HNE) antibody (Atto 488)
HNE WB, ELISA, ICC, IF Host: Mouse Monoclonal 12F7 Atto 488

Application Notes

IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Chien, Wen, Cheng, Tsai, Chiang, Chien: "Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 8, (2020) (PubMed).

Vairappan, Sundhar, Srinivas: "Resveratrol Restores Neuronal Tight Junction Proteins Through Correction of Ammonia and Inflammation in CCl4-Induced Cirrhotic Mice." in: Molecular neurobiology, Vol. 56, Issue 7, pp. 4718-4729, (2019) (PubMed).

Sapkota, Gaire, Cho, Jeon, Kwon, Jang, Kim, Ryu, Choi: "Eupatilin exerts neuroprotective effects in mice with transient focal cerebral ischemia by reducing microglial activation." in: PLoS ONE, Vol. 12, Issue 2, pp. e0171479, (2017) (PubMed).

Kagaya, Sasaki, Kino, Taniguchi, Kuraishi, Andoh: "Involvement of oxidative stress in increased peripheral nerve firing during spontaneous dysesthesia in a mouse model of ischemia-reperfusion." in: Neuroscience letters, Vol. 631, pp. 109-14, (2016) (PubMed).

Zhang, Song, Pang, Zhang, Wan, Yuan, Wu, Liu: "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." in: World journal of gastroenterology : WJG, Vol. 21, Issue 14, pp. 4195-209, (2015) (PubMed).

Polverino, Doyle-Eisele, McDonald, Wilder, Royer, Laucho-Contreras, Kelly, Divo, Pinto-Plata, Mauderly, Celli, Tesfaigzi, Owen: "A novel nonhuman primate model of cigarette smoke-induced airway disease." in: The American journal of pathology, Vol. 185, Issue 3, pp. 741-55, (2015) (PubMed).

Duong, Chami, McMahon, Fong, Dennis, Freedman, Witting: "Pre-treatment with the synthetic antioxidant T-butyl bisphenol protects cerebral tissues from experimental ischemia reperfusion injury." in: Journal of neurochemistry, Vol. 130, Issue 6, pp. 733-47, (2014) (PubMed).

Haberman, Tickle, Dexheimer, Kim, Tang, Karns, Baldassano, Noe, Rosh, Markowitz, Heyman, Griffiths, Crandall, Mack, Baker, Huttenhower, Keljo, Hyams, Kugathasan, Walters, Aronow, Xavier, Gevers et al.: "Pediatric Crohn disease patients exhibit specific ileal transcriptome and microbiome signature. ..." in: The Journal of clinical investigation, Vol. 124, Issue 8, pp. 3617-33, (2014) (PubMed).

Guo, Tong, Xi, Yang, Dong, Wen: "Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats." in: Journal of ethnopharmacology, Vol. 144, Issue 3, pp. 768-74, (2012) (PubMed).
Target

HNE (4-Hydroxynonenal (HNE))

Alternative Name

4 Hydroxynonenal (HNE Products)

Target Type

Chemical

Background

Synonyms: 4-Hydroxy-2-Nonenal, 4Hydroxynonenal, 4-Hydroxynonenal, 4 HNE, 4HNE, 4-HNE, E-4-Hydroxynonenal-dimethylacetal, 4-HNE-DMA.
Background: Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress 4-HNE has been hypothesized to play a key role in cell signal transduction, in a variety of pathways from cell cycle events to cellular adhesion.