BrdU antibody (FITC)

Catalog No. ABIN336862

Product Details anti-BrdU Antibody (FITC)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Bromodeoxyuridine.

Immunogen: BrdU conjugated to KLH

Clone: BrdU 206

Isotype: IgG1 kappa

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. (For staining of FFPE tissue sections, incubate with 4 N HCl for 20 minutes at RT, followed by treatment with proteolytic digestion for 10 minutes at RT. (Please refer to our Trypsin (AR-6541), Pronase (AR-6542) or pepsin (AR-6543) data sheets. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Liver of experimental animal injected with BrdU or cell line grown in presence of BrdU. Cellular Localization: Nuclear.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a syn

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BRDU (BrdU Products)

Target Type: Chemical