EpCAM antibody

Catalog No. ABIN336888

The Mouse Monoclonal anti-EpCAM antibody (Clone ESA214) (ABIN336888) specifically detects EpCAM in IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for EpCAM antibody (ABIN336888)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ESA214

Product Details anti-EpCAM Antibody

Specificity: Molecular Weight: 40 kDa.

Immunogen: Small cell lung carcinoma

Isotype: IgG1 kappa

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. .(Antigen retriever like pepsin enhances the staining of FFPE tissue, please see data sheet for pepsin cat. # AR-6543. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Breast carcinoma. Cellular Localization: Cell membrane.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The concentrated antibodies (Protein A purified) are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Epithelial is a transmembrane glycop

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM