GNAI1 antibody

Catalog No. ABIN337268

The Mouse Monoclonal anti-GNAI1 antibody is suitable to detect GNAI1 in samples from Human, Mouse, Rat, Cow and Guinea Pig. It has been validated for WB, IHC and IHC (p).

Quick Overview for GNAI1 antibody (ABIN337268)

Target: GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This GNAI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R4-5

Product Details anti-GNAI1 Antibody

Specificity: Recognizes Rat Gai1. Does not cross-react with Transducin, Gao, Gai2, Gai3, or Gas. ADP-ribosylation of ai1 proteins does not alter the reactivity. Species cross-reactivity: mouse, bovine, guinea pig and human.

Purification: Protein A purified

Immunogen: Partially purified Gai1 protein from rat brain (39-42kD). Cellular Localization: Cell membrane.

Type of Immunogen: Protein fraction

Isotype: IgG2b

Application Details

Application Notes: Approved: IHC, IHC-P (1:50), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Positive control: IMR-5 cells, brain.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GNAI1

Target: GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))

Alternative Name: GNAI1 / Gi

Background: Name/Gene ID: GNAI1

Synonyms: GNAI1, Gi1 protein alpha subunit, Gi

Gene ID: 2770

UniProt: P63096

Pathways: G-protein mediated Events