GNAI1 antibody (Center)

Catalog No. ABIN2856077

Product Details anti-GNAI1 Antibody

Target: GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (100 %), Rat (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to G protein alpha Inhibitor 1 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1)
G protein alpha Inhibitor 1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human G protein alpha Inhibitor 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A431 , HeLaS3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GNAI1

Target: GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))

Alternative Name: G Protein alpha Inhibitor 1 (GNAI1 Products)

Synonyms: AU046200 antibody, Gialpha1 antibody, Gnai-1 antibody, gnai1 antibody, Gi antibody, BPGTPB antibody, zgc:63957 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting 1 antibody, G protein subunit alpha i1 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 S homeolog antibody, guanine nucleotide-binding protein G(i) subunit alpha-1 antibody, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 antibody, Gnai1 antibody, gnai1.S antibody, GNAI1 antibody, LOC397505 antibody, gnai1 antibody

Background: Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase, Go, a protein abundant in brain (GNAO1, MIM 139311), and transducin-1 (GNAT1, MIM 139330) and transducin-2 (GNAT2, MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218], Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein, see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]

Molecular Weight: 40 kDa

Gene ID: 2770

Pathways: G-protein mediated Events