GNAI1 antibody (AA 146-195)

Catalog No. ABIN6738969

The Rabbit Polyclonal anti-GNAI1 antibody is suitable to detect GNAI1 in samples from Human, Mouse, Rat, Cow, Pig, Dog, Rabbit, Zebrafish (Danio rerio), Drosophila melanogaster, Hamster, Monkey and Bat. It has been validated for WB.

Quick Overview for GNAI1 antibody (AA 146-195) (ABIN6738969)

Target: GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Rabbit, Zebrafish (Danio rerio), Drosophila melanogaster, Hamster, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GNAI1 Antibody

Binding Specificity: AA 146-195

Specificity: Human GNAI1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Horse, Guinea pig (92%) Chicken, Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of human GNAI1 (P63096, NP_002060). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Drosophila (100%), Horse, Opossum, Guinea pig (92%), Turkey, Zebra finch, Chicken, Platypus, Xenopus (85%), Zebrafish, Nematode (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GNAI1

Target: GNAI1 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1))

Alternative Name: GNAI1 / Gi

Background: Name/Gene ID: GNAI1

Synonyms: GNAI1, Gi1 protein alpha subunit, Gi

Gene ID: 2770

NCBI Accession: NP_002060

UniProt: P63096

Pathways: G-protein mediated Events