CD4 Molecule (CD4) antibody

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (GK1-5)

Host: Rat

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunocytochemistry (ICC)

Catalog no.: ABIN343704

Additional Information

Alternative name: CD4

Immunogen: Mouse CTL clone V4 cells

Format: Azide Free

Isotype: IgG2b  (Matching secondary antibodies)

Clone: GK1-5

Description: CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. Inits extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like proteintyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cellmembrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region inCD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) andother ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptorinvolved in immune response (co-receptor activity in binding to MHC class II molecules) andHIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cellselection and signal transduction. Defects in antigen presentation (MHC class II) causedysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue andorgans (SCID immunodeficiency).

Specificity: The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4transmembrane glycoprotein (55 kDa).

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 1 µg/million cells. Immunoprecipitation - Recommended dilution: 1-2 µg/100-500 µg of protein in 1 ml lysate. Immunocytochemistry - Recommended dilution: 1-4 µg/ml. Functional Application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Wu, Antica, Johnson et al.: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992 (PubMed).

Dialynas, Wilde, Marrack et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity." in: Immunological reviews, Vol. 74, pp. 29-56, 1983 (PubMed).

Wilde, Marrack, Kappler et al.: "Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2178-83, 1983 (PubMed).

Dialynas, Quan, Wall et al.: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, 1983 (PubMed).

Godfrey, Kennedy, Gately et al.: "IL-12 influences intrathymic T cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2729-35, 1994 (PubMed).

Gavett, Chen, Finkelman et al.: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, 1994 (PubMed).

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, 1997 (PubMed).

Felix, Donermeyer, Horvath et al.: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." in: Nature immunology, Vol. 8, Issue 4, pp. 388-97, 2007 (PubMed).

Yi, Zhen, Zeng et al.: "Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice." in: Transplantation, Vol. 85, Issue 8, pp. 1167-74, 2008 (PubMed).

Hu, Watson, Zhang et al.: "Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6593-603, 2008 (PubMed).