CD81 antibody (CD81 Molecule) (PE)

Details for Product anti-CD81 Antibody No. ABIN343728, Supplier: Log in to see
Antigen
  • Tapa-1
  • Tapa1
  • Tspan28
  • CVID6
  • S5.7
  • TAPA1
  • TSPAN28
  • CD81 molecule
  • zgc:92411
  • CD81 antigen
  • Cd81 molecule
  • CD81
  • zgc:92411
  • Cd81
Alternatives
anti-Mouse (Murine) CD81 antibody for Flow Cytometry
Reactivity
Human, Cat (Feline), Rabbit
279
188
56
35
34
27
27
22
19
5
3
3
1
1
1
1
Host
Mouse
209
76
50
30
21
7
5
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD81 antibody is conjugated to PE
48
45
30
11
11
9
8
8
6
5
5
5
5
5
4
4
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
327
129
103
95
87
73
60
48
41
24
13
8
7
5
4
3
3
2
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen MOLT-4 (human T-ALL cell line)
Clone M38
Isotype IgG1
Specificity The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD81 (CD81 Antibody Abstract)
Background CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells –, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Inositol Metabolic Process, Hepatitis C
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Product cited in: Escola, Kleijmeer, Stoorvogel, Griffith, Yoshie, Geuze: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, 1998 (PubMed).

Imai, Kakizaki, Nishimura, Yoshie: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, 1995 (PubMed).

Imai, Yoshie et al.: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, 1994 (PubMed).

Fukudome, Furuse, Imai, Nishimura, Takagi, Hinuma, Yoshie et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, 1992 (PubMed).