Collagen IV antibody

Catalog No. ABIN344815

This Rabbit Polyclonal antibody specifically detects Collagen IV in ELISA, IHC, IHC (p) and IF. It exhibits reactivity toward Mouse.

Quick Overview for Collagen IV antibody (ABIN344815)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-Collagen IV Antibody

Specificity: Recognizes mouse collagen Type IV: 100 % , mouse Types I, II, III: <0.1 %, Human Types IV, V: <0.1 %, Mouse Fibronectin: <0.1 %, Mouse Laminin: <0.1 %. Determined by RIA. Species cross-reactivity: reacts weakly with human.

Purification: Immunoaffinity purified

Immunogen: Native Collagen Type IV from mouse EHS tumor.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:100 - 1:400), IF (1:40 - 1:160), IHC, IHC-P

Usage: Suitable for use in Immunofluorescence, Immunohistochemistry and ELISA. Immunofluorescence: 1:40-1:160. Immunohistochemistry (paraffin): 1:250-1:1000. (Recommended Immunohistochemistry fixatives: AFA, buffered formalin (5-10 % ) or Bouin's). ELISA: 1:100-1:400.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen IV