Collagen IV antibody (AA 1571-1669)

Catalog No. ABIN707396

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Binding Specificity: AA 1571-1669

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Collagen IV antibody (ABIN707396)

Sato, Coburn: "Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells." in: PLoS neglected tropical diseases, Vol. 11, Issue 7, pp. e0005830, (2017) (PubMed).

Wu, Zhang, Ma, Zhang, Qin: "The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats." in: Molecular biology reports, Vol. 39, Issue 9, pp. 9085-93, (2012) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen 4 (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody

Background:

Synonyms: ICH, HANAC, POREN1, arresten, Collagen alpha-1(IV) chain, COL4A1

Background: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

Gene ID: 1282

UniProt: P02462