Collagen IV antibody

Catalog No. ABIN344821

This Rabbit Polyclonal antibody specifically detects Collagen IV in WB, ELISA, IHC, IHC (fro) and IP. It exhibits reactivity toward Human.

Quick Overview for Collagen IV antibody (ABIN344821)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Product Details anti-Collagen IV Antibody

Specificity: Recognizes human Collagen Type IV. Typically less than 1 % cross-reactivity against other types of collagens was detected by ELISA against purified standards. Reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reactivity with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Purification: Immunoaffinity purified

Immunogen: Collagen Type IV from human and bovine placenta.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:4000 - 1:8000), IHC, IHC-Fr, IP, WB (1:5000 - 1:10000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 1:4000-1:8000. Western Blot: 1:5000-1:10000. Immunohistochemistry (Frozen sections): 1:50-1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: BBS,  pH 8.0, 0.005 M EDTA, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen IV