HIF1A antibody (AA 432-528)

Catalog No. ABIN2852421

The Mouse Monoclonal anti-HIF1A antibody is suitable to detect HIF1A in samples from Human, Mouse, Rat, Rabbit, Monkey and Sheep. It has been validated for WB, IHC, IP, ELISA, ICC, IF, FACS, ChIP and EMSA.

Quick Overview for HIF1A antibody (AA 432-528) (ABIN2852421)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivity: Human, Mouse, Rat, Rabbit, Monkey, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: H1alpha67

Product Details anti-HIF1A Antibody

Binding Specificity: AA 432-528

Specificity: Reacts with human HIF-1 alpha

Cross-Reactivity: Mouse (Murine), Cow (Bovine), Rabbit, Sheep (Ovine)

Cross-Reactivity (Details): Cross reacts with proteins from monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret.

Purification: Purified (protein A)

Immunogen: Fusion protein containing amino acids 432-528 of human HIF-1alpha

Isotype: IgG2b

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ChIP(1:10 - 1:500) GS(1:1 - 1:100) ICC/IF(1:50) IHC(1:20 - 1:50) IP(1:10 - 1:500) WB: (1:500)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: Hypoxia-inducible factor 1-alpha

Background: HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis.This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 alpha is involved in B-1 B cell homeostasis, Notch signaling pathway and many other biological processes.

Gene ID: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect