PADI4 antibody (Peptidyl Arginine Deiminase, Type IV) (N-Term)

Details for Product anti-PADI4 Antibody No. ABIN347069, Supplier: Log in to see
Antigen
  • PAD
  • PAD4
  • PADI5
  • PDI4
  • PDI5
  • Pad4
  • Pdi4
  • peptidyl arginine deiminase, type IV
  • PADI4
  • Padi4
Epitope
N-Term
18
12
9
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
103
14
13
2
2
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
67
23
16
Clonality
Polyclonal
Conjugate
This PADI4 antibody is un-conjugated
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
78
33
31
21
19
13
7
6
5
5
2
2
1
1
1
1
1
Options
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Immunogen Synthetic peptide from N terminal part of human PADI4 protein
Isotype IgG
Specificity Reacts with PADI4 from human, mouse and rat
Cross-Reactivity (Details) No cross reaction with the other isoforms.
Purification Antiserum
Alternative Name Protein-Arginine Deiminase Type-4 (PADI4 Antibody Abstract)
Background PADI4 is the enzyme that catalyze the citrullination or the deimination of arginine on proteins like Histone H3.
Gene ID 23569
UniProt Q9UM07
Application Notes

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA(1:1000 - 1:5000) ICC(1:50 - 1:100) IP(1:20 - 1:100) WB(1:200 - 1:2000)

Restrictions For Research Use only
Format Lyophilized
Reconstitution Must be reconstituted in distilled water.
Storage 4 °C/-20 °C
Storage Comment Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.
Expiry Date 24 months
Background publications Wang, Wysocka, Sayegh, Lee, Perlin, Leonelli, Sonbuchner, McDonald, Cook, Dou, Roeder, Clarke, Stallcup, Allis, Coonrod: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).

Nakashima, Hagiwara, Ishigami, Nagata, Asaga, Kuramoto, Senshu, Yamada: "Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1alpha,25-dihydroxyvitamin D(3)." in: The Journal of biological chemistry, Vol. 274, Issue 39, pp. 27786-92, 1999 (PubMed).