PCK1 antibody

Catalog No. ABIN349649

The Rabbit Polyclonal anti-PCK1 antibody has been validated for WB. It is suitable to detect PCK1 in samples from Zea mays, Arabidopsis thaliana, Barley, Nicotiana tabacum, Phaeodactylum tricornutum and Synechocystis. There are 3+ publications available.

Quick Overview for PCK1 antibody (ABIN349649)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Zea mays, Arabidopsis thaliana, Barley, Nicotiana tabacum, Phaeodactylum tricornutum, Synechocystis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCK1 Antibody

Characteristics: Expected / apparent Molecular Weight of the Antigene: 110 / 105 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At1g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4)

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with standard ECL (WB).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-PCK1 antibody (ABIN349649)

Chang, Jeon, Gu, Pack, Jin: "Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase." in: Bioprocess and biosystems engineering, (2013) (PubMed).

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

Wostrikoff, Clark, Sato, Clemente, Stern: "Ectopic expression of Rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation." in: Plant physiology, Vol. 160, Issue 1, pp. 419-32, (2012) (PubMed).

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: Phosphoenolpyruvate Carboxylase

Background: AGI Code: At1g53310, At1g14940
PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3

Molecular Weight: expected: 110 kDa, apparent: 105 kDa

NCBI Accession: NP_177043

UniProt: Q84VW9, Q9MAH0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis