PCK1 antibody

Catalog No. ABIN349649

There are 3+ publications for this product available. The Rabbit Polyclonal anti-PCK1 antibody is suitable to detect PCK1 in samples from Zea mays, Saccharum, Pineapple, Oryza sativa, Megathyrsus maximus, Panicum coloratum, Petrosimonia nigdeensis, Phaeodactylum tricornutum, Pinus strobus, Salsola grandis, Salsola lanata, Salsola laricifolia, Salsola tragus, Sorghum, Soybean, Synechocystis, Thalassiosira weisflogii, Zostera muelleri, Cenchrus ciliaris, Panicum antidotale, Nicotiana tabacum, Mesembryanthemum crystallinum, Lupine, Leptochloa fusca, Kochia prostrata, Jatropha curcas, Cyanthobasis fruticulosa, Chromera velia, Chloris gayana, Chenopodium quinoa, Barley and Arabidopsis thaliana. It has been validated for WB and IL.

Quick Overview for PCK1 antibody (ABIN349649)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Zea mays, Saccharum, Pineapple, Oryza sativa, Megathyrsus maximus, Panicum coloratum, Petrosimonia nigdeensis, Phaeodactylum tricornutum, Pinus strobus, Salsola grandis, Salsola lanata, Salsola laricifolia, Salsola tragus, Sorghum, Soybean, Synechocystis, Thalassiosira weisflogii, Zostera muelleri, Cenchrus ciliaris, Panicum antidotale, Nicotiana tabacum, Mesembryanthemum crystallinum, Lupine, Leptochloa fusca, Kochia prostrata, Jatropha curcas, Cyanthobasis fruticulosa, Chromera velia, Chloris gayana, Chenopodium quinoa, Barley, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-PCK1 Antibody

Purpose: Anti-PEPC | Phosphoenolpyruvate carboxylase

Cross-Reactivity (Details): Ananas comosus, Arabidopsis thaliana, Cenchrus ciliaris, Chenopodium quinoa, Chloris gayana, Chromera velia, Cyanthobasis fruticulosa, Glycine max, Hordeum vulgare, Jatropha curcas, Kochia prostrata, Leptochloa fusca, Lupinus sp. , Megathyrsus maximus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Panicum antidotale, Panicum coloratum, Petrosimonia nigdeensis, Pinus strobus, Saccharum spp. hybrid clone C91-301, Salsola lanata, Salsola laricifolia,Salsola grandis, Salsola tragus Sorghum bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), Pinus strobus, Thalassiosira weissfloggi, Zea mays, Zostera muelleri
Not reactive in: Methanothermobacter thermautotrophicus

Predicted Reactivity: Amaranthus sp.,Brassica napus,Cucumis sativus (PEPC1,PEPC2,PEPC3),Flaveria bidentis,Flaveria trinervia,Lupinus albus,Mammillaria thornberi,Manihot esculenta,Manihot obovata,Medicago sativa,Morinda citrifolia,Nannochloropsis gaditana CCMP526,Nopalea gaumeri,Opuntia macbridei,Pachycereus pringlei,Pachycereus hollianus,Pisum sativa,Phaseolus vulgaris,Phragmites australis,Populus sp.,Saccharum spp,Solanum tuberosum,Spinacia oleracea,Streptanthus tortuosus,Triticum aestivum,algae,diatoms: Thalassiosira pseudonana,other species: Salmonella sp.,Schiedea hookeri,Shigella sp. Schiedea sarmentosa,Solanum lycopersicum,Streptanthus farnsworthianus,Tacinga saxatilis,Yersinia sp. Vibrio sp.,Quercus sp.

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0

Application Details

Application Notes: 1 : 500 (IL), 1: 1000 - : 10 000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation.

References for anti-PCK1 antibody (ABIN349649)

Chang, Jeon, Gu, Pack, Jin: "Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase." in: Bioprocess and biosystems engineering, (2013) (PubMed).

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

Wostrikoff, Clark, Sato, Clemente, Stern: "Ectopic expression of Rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation." in: Plant physiology, Vol. 160, Issue 1, pp. 419-32, (2012) (PubMed).

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: Phosphoenolpyruvate Carboxylase

Background:

Background: PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3

Additional information: PEPC is especially prone to proteolysis. To avoid artifacts, we use chymotrypsin, as recommended by Plaxton (2019), in addition to the Roche protease inhibitor cocktail.

Molecular Weight: 110 | 105 kDa

Gene ID: 841765

NCBI Accession: NP_177043

UniProt: Q9MAH0, Q84VW9

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis