Mouse anti-Human IgG (Fab Region) Antibody (PE)

Details for Product No. ABIN349709, Supplier: Log in to see
Antigen
Epitope
Fab Region
4684
2573
1882
1039
1015
825
305
272
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
2780
2759
2347
1724
1436
778
607
426
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Mouse
6750
4661
1834
811
684
394
88
52
51
9
8
5
5
4
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
PE
2183
1862
1718
1362
765
675
521
297
294
284
274
268
217
186
178
117
102
79
69
58
54
40
35
26
23
22
19
19
19
19
19
16
15
15
15
15
15
15
14
13
12
11
11
10
10
9
9
9
9
9
9
9
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
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Immunogen Purified human IgG.
Clone 4A11
Isotype IgG2b
Specificity The antibody 4A11 reacts with Fab fragment of human IgG.
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Background Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Molecular Weight 150 kDa
Research Area Immunology, Secondary Antibodies
Application Notes The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 6 µg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Comment

Excitation/Emission wavelength: 496 nm/576 nm

Restrictions For Research Use only
Format Liquid
Reconstitution No reconstitution is necessary.
Concentration 0.1 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Fab Region) Antibody (PE) (ABIN349709) Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).