Unc-51 Like Kinase 1 (C. Elegans) (ULK1) (Internal Region) antibody

Antigen: Unc-51 Like Kinase 1 (C. Elegans) (ULK1)

Synonyms: ULK1, Unc51.1, AU041434, mKIAA0722

Binding Site: Internal Region

Clonality: Polyclonal

Host: Sheep

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350008

Additional Information

Alternative name: ULK1 (ATG1)

UniProt: O75385

Immunogen: A synthetic peptide as a part of Sheep antibody to human, rat, mouse serine/threonine-protein kinase ULK1 (ULK1, ATG1, UNC51): IgG conjugated to an immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBUNIT: Interacts with GABARAP and GABARAPL2. Also known as: Unc-51-like kinase 1, ATG1, UNC51, Unc51.1.

Specificity: Appears to be specific for ULK1.

Application Details

Application Notes: IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Autophagy

Restrictions: For Research Use only

Publications

Kuroyanagi, Yan, Seki et al.: "Human ULK1, a novel serine/threonine kinase related to UNC-51 kinase of Caenorhabditis elegans: cDNA cloning, expression, and chromosomal assignment." in: Genomics, Vol. 51, Issue 1, pp. 76-85, 1998 (PubMed).

Okazaki, Yan, Yuasa et al.: "Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation." in: Brain research. Molecular brain research, Vol. 85, Issue 1-2, pp. 1-12, 2001 (PubMed).

Tomoda, Kim, Zhan et al.: "Role of Unc51.1 and its binding partners in CNS axon outgrowth." in: Genes & development, Vol. 18, Issue 5, pp. 541-58, 2004 (PubMed).

Young, Chan, Hu et al.: "Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes." in: Journal of cell science, Vol. 119, Issue Pt 18, pp. 3888-900, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Wissing, Jänsch, Nimtz et al.: "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 3, pp. 537-47, 2007 (PubMed).

Chan, Kir, Tooze: "siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy." in: The Journal of biological chemistry, Vol. 282, Issue 35, pp. 25464-74, 2007 (PubMed).