ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae))

Details for Product anti-ATG5 Antibody No. ABIN350087, Supplier: Log in to see
Antigen
  • APG5
  • APG5-LIKE
  • APG5L
  • ASP
  • hAPG5
  • ATG5
  • CG1643
  • DmAtg5
  • Dmel\\CG1643
  • atg5
  • 2010107M05Rik
  • 3110067M24Rik
  • AW319544
  • Apg5l
  • Atg5l
  • C88337
  • Paddy
  • apg5l
  • zgc:100934
  • ATATG5
  • AUTOPHAGY 5
  • MKP11.20
  • MKP11_20
  • autophagy related 5
  • Autophagy-specific gene 5
  • ATG5 autophagy related 5 homolog (S. cerevisiae)
  • APG5 autophagy 5-like
  • similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12
  • autophagy protein 5
  • ATG5
  • Atg5
  • atg5
  • LOC100227318
  • LOC100342807
  • APG5
Alternatives
anti-Human ATG5 antibody for Western Blotting
Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This ATG5 antibody is un-conjugated
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Application
Immunohistochemistry (IHC), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen A synthetic peptide from human autophagy protein 5 (ATG5, ASP, APG5, APG5L, hAPG5) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in mouse, xenopus and chicken.
Isotype IgG
Specificity Specific for ATG5.
Alternative Name ATG5 (ATG5 Antibody Abstract)
Background ATG5 is an important element forautophagy and may also play an important role in the apoptotic process. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. While the mRNA is ubiquitously transcribed its expression is highly upregulated in apoptotic cells. Also known as: APG5-like, ATG5, ASP, APG5, APG5L, hAPG5, apoptosis-specific protein.
Pathways Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy
Application Notes Use at a concentration of 10-50 µg/ml.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Simonsen, Birkeland, Gillooly, Mizushima, Kuma, Yoshimori, Slagsvold, Brech, Stenmark: "Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes." in: Journal of cell science, Vol. 117, Issue Pt 18, pp. 4239-51, 2004 (PubMed).

Schmeiser, Armstrong, Hammond, Grand: "Assignment of the yeast APG5 human homologue APG5L to chromosome band 6q21 by fluorescence in situ hybridisation." in: Cytogenetics and cell genetics, Vol. 87, Issue 3-4, pp. 213-4, 2000 (PubMed).

Mizushima, Sugita, Yoshimori, Ohsumi: "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 33889-92, 1999 (PubMed).

Hammond, Brunet, Johnson, Parkhill, Milner, Brady, Gregory, Grand: "Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast." in: FEBS letters, Vol. 425, Issue 3, pp. 391-5, 1998 (PubMed).

Grand, Milner, Mustoe, Johnson, Owen, Grant, Gregory: "A novel protein expressed in mammalian cells undergoing apoptosis." in: Experimental cell research, Vol. 218, Issue 2, pp. 439-51, 1995 (PubMed).

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